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please answer the following questions. Attached is reference for the pdf use the attached documents to answer the following questions:
Question 1: Dr. Heme has a leukemia patient who moved to the U.S. from the mountains of Peru. The physician strongly believes that the patient would do well with a bone marrow transplant. The patient has no relatives nearby but told the physician that he believes that his ancestors are from Spain. Dr. Heme then began to look for a matched donor using the bone marrow registry with potential donors from Spain. What is wrong with Dr. Heme’s approach? Discuss the limitation of the current bone marrow registry.
Question 2 : You have added your DNA to the bone marrow registry. After a few years you receive a phone call that you are a match for someone. A non-MLS friend of yours is concerned that this might compromise your reserve of hematopoietic stem cells (HSCs). Based on what you have learned in this unit how can you negate your friend’s statement?
Question 3 : The transplant physician is about to infuse mobilized peripheral blood to a leukemia patient, however Dr. Oncologist is concerned that cells from the donor may not repopulate the patient’s long-term blood/immune system. To alleviate Dr. Oncologist’s concern, you offered to phenotype the donor’s cells by flow cytometry. You have determined that most of the cells are phenotypically hematopoietic cells. What is the phenotype of the cells that leads you to your conclusion? What will you tell Dr. Oncologist to alleviate her concern? What test can be performed, usually in a research environment, to provide additional information for the oncologist?
Question 4 : A bone marrow donor has provided mobilized peripheral blood for an allogenic transplant for leukemia. Phenotyping the donor mobilized peripheral blood demonstrated the predominance of CD133+/CD34-/Lin-. What do you think would be the outcome of the transplant? Begin with the early phase (within the first 2 weeks) to long-term (greater than 1 year). Explain your answer.
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